Nucleotide sequence of Chlamydomonas moewusii chloroplastic tRNA
نویسنده
چکیده
Following the physical mapping of Chlamydomonas moewusii chJoroplastic DNA (1), we selected clones on the basis of their hybridization with positively light-regulated transcripts from the same organism (2, 3). One such clone containing a 13.5 kb insert was subcloned and a SaR-Xbal 2.4 kb fragment sequenced. It contained, among other features, a 1,689 bp ORF 563 (4) similar to ORF 513 from the chloroplastic genome of Marchantia polymorpha (5) and a (tgt) tRNA spanning nucleotides —602 to —528 with respect to ORF 563 initiation codon. For comparison, in M. polymorpha a similar (tgt) tRNA* is located about 26,000 bp downstream from ORF 513, indicating evolutionary rearrangements; moreover in this organism a (ttg) tRNA"" can be found between -245 and -176 (5). ACKNOWLEDGEMENTS
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